Sensitization

Sensitization efficacy is assessed using different techniques:

Sensitization tests are useful to assess whether the product in exam may cause adverse effects involving immunological mechanisms; in vitro methods are currently under validation process. The human Cell Line Activation Test (h-CLAT) quantifies the induction of protein markers on the surface of THP-1 cell lines after exposure to the test product. Only for Medical devices and Nutrition products the officially accepted animal test methods include the Mouse Local lymph Node Assay (LLNA), the Guinea Pig Maximisation Test and the Buehler test.