A set of in vitro testing for the reproduction risk assessment is performed, basing on assisted reproduction techniques, such as the in vitro oocyte maturation and fecundation assays on bovine model.
In vitro maturation test:
an assay reproducing the process of oocyte maturation, which corresponds to the pre-ovulatory in vivo phase that allows to highlight specific toxic effects on oocytes (INVITTOX protocol n ° 129).
In vitro fertilization test:
by exposing oocytes and sperm to the substances to be tested. Allows to evaluate toxic effects on oocytes an oocyte-sperm interaction during fertilization (DB-ALM Protocol No. 128).